Skip to content - navigation

Home » Vein to Vein » Donations » Component Preparation

Preparation of Cryoprecipitate and Cryosupernatant Plasma

Canadian Blood Services produces two products from Fresh Frozen Plasma, LR

  • Cryosupernatant, LR (Leukocytes reduced), which is also known as CSP, LR
  • Cryopreciptate, LR (Leukocytes reduced), which is also known as Cryo, LR

Following the implementation of the Buffy Coat Production Method (BCPM), these components produced from CPD Frozen Plasma will also be available:

  • CPD Cryosupernatant Plasma
  • Cryoprecipitate

Preparation of CSP, LR and Cryo, LR

Cryoprecipitate is so named because it precipitates when frozen plasma is thawed at low temperatures. Below is a simple description of how Cryoprecipitate, LR and Cryosupernatant, LR is prepared from CP2D whole blood. Leukofiltration is not shown.

Step 1

 The whole blood donation is centrifuged to separate red cells from plasma.

 

Step 2

 Approximately 190-260 mL of donor plasma is expressed into the satellite bag. The RBC unit is sealed and stored at 1-6o C for varying shelf lives. Not shown is the AS-3 container should the RBC be made into an AS-3 RBC.

 

Step 3

 Donor plasma is rapidly frozen within eight hours of collection to preserve Factor VIII.

 

Step 4

 

 Donor plasma is thawed slowly at 1-6°C to the slush stage. Plasma is then centrifuged to separate the cryoprecipitated portion (i.e., cryoprecipitate) from the liquid portion (cryosupernatant plasma).

 
Step 5
 All but ~10 mL of plasma is expressed into the final satellite bag. The three mL of Cryo remains in a plasma volume of about 5-15 mL.

Plasma separated from the Cry is know as Cryosupernatant Plasma and can be < href="/resources/books/vein-vein/pretransfusion/storage-blood-componants">stored frozen for u to 12 month t temperatures less than minus 20OC. (While at Canadian Blood Services)

Cryo i href="/resources/books/vein-vein/pretransfusion/storage-blood-componants">stored frozen for u to 12 month t 20OC or colder (whil store at CBS).

 

Preparat on of CPD Cryosupernatant Plasma and CPD Cryoprecipitate

CPD Cryos pernatant Plasma is th newly froze roduc obtained following th production of CPD Cryo using the Buffy Coat Production Method (BCPM).

Below is simple schematic of how CPD Cryoprecipitate and CPD Cyrosupernan Plasma is prepared fro CPD Whole Blood. 

Text will be replaced with image replacement