Transfusion-Associated Graft-vs- Host Disease (TA-GVHD)

Description

Transfusion-associated graft-versus-host disease (TA-GVHD) is a life-threatening complication that may occur in immunocompromised patient following the transfusion of cellular blood components (red cell concentrates, platelet concentrates, granulocyte concentrates).

In rare circumstances, TA-GVHD may occur in immunocompetent patients who receive HLA-matched blood components or transfusions from first-degree family members (namely, parents, children, siblings) due to shared specificities at the major histocompatibility complex. For example, if the patient is heterozygous at an HLA locus and the donor is homozygous for one of the corresponding loci, the immunocompetent patient will not recognize the donor as foreign.

Incidence

The incidence of TA-GVHD is unknown but rare.

Clinical Presentation - Transfusion-Associated Graft-vs- Host Disease (TA-GVHD)

The clinical syndrome consists of fever, skin rash, diarrhea, hepatic dysfunction, and bone marrow aplasia, typically appearing eight to ten days after transfusion.
The outcome has a high fatality rate, with hemorrhage and infection as the most common causes of death.

Mechanism

TA-GVHD results when transfused T lymphocytes present in cellular blood components engraft, multiply, and react against the tissues of the recipient.

Investigation - Transfusion-Associated Graft-vs- Host Disease (TA-GVHD)

Transfusion services should have clear policies describing the required investigation transfusion complications.

An investigation will only be initiated if the treating physician is aware of this possibility in a susceptible patient with a clinical picture suggestive of Transfusion-Associated Graft vs. Host Disease (TA-GVHD).

The investigation begins with the confirmation of the presence of GVHD. This is a pathologic diagnosis requiring a skin or intestinal biopsy. If GVHD is present, further studies should be performed to confirm the engraftment of donor lymphocytes.

Reporting - Transfusion-Associated Graft-vs- Host Disease (TA-GVHD)

Overview

Documenting and reporting complications of blood transfusion involve many aspects and interrelationships. Policies and procedures will vary from site to site. Where applicable, please find examples of the types of reporting that are required.

Responsibilities of Medical and Nursing Staff

Physicians and nurses attending to patients who experience suspected transfusion complications should perform the following documentation and reporting functions:

  • Report suspected reactions immediately to the attending physician and transfusion service.
  • Document the patient's signs and symptoms and implicated donor units and send them to the transfusion service, as shown in this example from the National TTI Surveillance System (TTISS):
    - Canadian Transfusion Adverse Event Reporting Form

Note: Canadian Blood Services offers no endorsement of and assumes no liability for the currency, accuracy, or availability of any information on these sites.

  • Maintain records of the complication in the patient’s medical record, including the report of the investigation completed by the transfusion service.

Note: Documentation must be maintained for all transfusions, whether or not complications occur.

Responsibilities of the Transfusion Service

The transfusion service is responsible for several aspects of documenting and reporting transfusion reactions and complications. These include documenting and reporting:

  • results of transfusion reaction investigations to the attending physician;
  • accidents and errors to the hospital transfusion committee;
  • significant complications to the manufacturer and/or distributor;
  • significant complications to other authorities as specified by provincial or federal regulations.

The types of reactions that should be reported are provided in the Standards for Blood Safety and below (under Canadian Blood Services).

Responsibilities of Canadian Blood Services

Canadian Blood Services, the blood supplier in all Canadian provinces and territories except Quebec, receives reports of serious adverse reactions from transfusion services and reports them to Health Canada.

IMPORTANT: In Canadian Blood Services’ Circular of Information, review a detailed description about the reporting responsibilities and relationships between itself and transfusion services, including transfusion-transmissible diseases: Section A6. Reporting Serious Adverse Reactions.

Prevention - Transfusion-Associated Graft-vs- Host Disease (TA-GVHD)

Currently the only method of preventing transfusion-associated graft versus host disease (TA-GVHD) is to gamma irradiate cellular components at risk of causing TA-GVHD or destined for at risk recipients. Current techniques to leukoreduce cellular blood components are not adequate to prevent TA-GVHD

  • Irradiated blood is prepared by exposing the component to a source of gamma irradiation. To eliminate the proliferative capacity of leukocytes, the central midplane of the canister should receive 2500 cGy and the lowest dose delivered to any portion of the canister should be 1500 cGy.

Canadian Blood Services produces the following gamma irradiated products:

  • Red Blood Cell products, LR
  • Platelets, LR
  • Platelets Apheresis, LR

Gamma irradiation, in the doses recommended for the prevention of TA-GVHD, does not affect the function of platelets. However, it does result in some damage to the erythrocyte membrane so that the permitted storage date of red cell concentrates is 28 days following irradiation (or the usual expiry date, whichever is shorter). There is also a more rapid accumulation of potassium in the extracellular fluid of the red cell concentrates. For this reason, for neonates and young children, it is preferable to gamma irradiate red cell components as close to the time of transfusion as possible. In these patients, if the units have not been irradiated just prior to transfusion, removal of extracellular fluid, (to reduce risks associated with high plasma potassium), may be considered.

Transfusion-Associated Graft-vs- Host Disease (TA-GVHD): Further Reading

Note: Canadian Blood Services offers no endorsement of and assumes no liability for the currency, accuracy, or availability of any information on these sites.

  1. Food and Drug Administration. Gamma Irradiation of Blood Products. CBER, FDA 1993.
  2. Guidelines for Irradiation of Blood and Blood Components. New York State Council on Human Blood and Transfusion Services. June 9, 1993.
  3. Luban NL. Irradiation for neonatal and pediatric transfusion in: Herman JH, Manno CS. Pediatric Transfusion Therapy. Bethesda MD: AABB Press; 2002: 147-169.
  4. Luban NL. Prevention of transfusion-associated graft versus host disease by inactivation of T cells in platelet components. Semin Hematol 2001 Oct;38 (4 Suppl 11):34-45. [ Medline ].
  5. McMilin KD, Johnson RL. HLA homozygosity and the risk of related-donor transfusion-associated graft versus host disease. Transfus Med Rev 1993 Jan; 7(1):37-41. [ Medline ].
  6. Nollet KE, Holland PV. Toward a coalition against transfusion-associated GVHD. Transfusion 2004; 43 (12):1655-7. [ Full Text ] [ Medline ]
  7. Triulzi Darrell J. Transfusion Support in Solid-Organ Transplantation. Institute for Transfusion Medicine. Transfusion Medicine Update. April 2001.
  8. Webb IJ, Anderson KC. Transfusion-associated graft versus host disease. In: Popovsky MA, ed. Transfusion reactions, 2nd ed. Bethesda, MD: AABB Press; 2001. 171-82.
  9. Wong ECC, Irradiated Products in: Hillyer CD, Strauss RG, Luban NLC. Handbook of Pediatric Medicine. San Diego CA: Elsevier Academic Press; 2004: 101-112.